WebDetritylation Solution SR674-4 Page 1 / 17 Version 2.0 Revision Date 08/16/2024 Print Date 10/06/2024 SECTION 1. IDENTIFICATION Product name : Detritylation Solution … WebFigure 2. The detritylation mechanism. The 5'-DMT protecting group is removed by TCA (trichloroacetic acid) in the solvent dichloromethane (too concentrated a solution of TCA or too long of a detritylation …
Trichloroacetaldehyde modified oligonucleotides - ScienceDirect
Web1. Collect the tritylcation solution in 15-ml glass tubes after each step. It is helpful touse graduated tubes so that a uniform final volume of acetonitrile canbe attained before … Web4. Detritylation solution: 4 % Dicholoroacetic acid in 1,2-dichloroethane. 5. Activator solution: Per coupled phosphoramidite use 360 μL of a 0.3 M solution of 5-benzylthio-1H-tetrazole (BTT) in acetonitrile and dry it overnight over activated molecular sieve (3 Å) prior to synthesis. 6. Capping solutions: Cap A-solution: 0.5 M 4-(dimethylamino)- iphone ptt hardware bluetooth
Glen Report 22.19 - Technical Brief - Depurination
WebNov 2, 2024 · In some cases, using weaker acids during detritylation or reducing acid contact times can be beneficial to maintain low levels of depurination. 1 DCA is a milder acid than TCA, ... Capping is generally achieved using a solution containing acetic anhydride (Cap Mix A) and the catalyst N-methylimidazole (Cap Mix B), ... WebDepurination had to be controlled by adjusting the fluidics of the flow cell and by quenching the detritylation solution with oxidizer solution. For researchers trying to determine if their source of depurination is … WebSep 15, 2002 · The detritylation reaction goes on for 10 s and is followed by a wash with 120 µl of acetonitrile (CH 3 CN). To achieve the highest coupling efficiency, the ejection of 4.5 µl of phosphoramidite and 11.5 µl of tetrazole is performed twice at each coupling stage. iphone psw