Tg fabp10a:cfp-ntr
Web1 Jan 2024 · Tg(fabp10a:CFP-NTR);Tg(fabp10a:DsRed) adult fish. Mating tanks: large aquaculture tanks with dividers. Fine plastic mesh strainer (tea strainer). Squeeze bottle. … Web21 Oct 2013 · Hepatocytes were depleted by administration of metronidazole to Tg(fabp10a:CFP-NTR) animals. We traced the origin of regenerating hepatocytes using …
Tg fabp10a:cfp-ntr
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WebThe Tg(fabp10a:CFP-NTR) adult fish were treated with 5 mM Mtz only for 5 hours and subsequently treated with MS-275 from R19h to R4d. As observed in the larvae, Bhmt … Web4 Jul 2014 · Similar to that observed in the larvae, the CFP and dim mCherry coexpressing cells were detected both in the MTZ- and EtOH/MTZ-treated regenerating livers of 12-month-old [Tg(fabp10a:CFP-NTR) gt1; Tg(Tp1:mCherry) jh11] adult fish at 3 days-post-ablation (dpa) (Fig. 2E″,F″, insets with arrows) with markedly elevated deposition of type I collagen …
Web42 rows · Tg(fabp10a:CFP-NTR) Previous Names. Tg(fabp10:CFP-Eco.NfsB) Tg(fabp10:CFP-NTR) Tg(fabp10a:CFP-Eco.NfsB) Tg(l-fabp:CFP-NTR) Type … WebTo ablate hepatocytes, Tg(fabp10a:CFP-NTR) larvae were treated with 10 mM metronidazole (Mtz) in egg water supplemented with 0.2% dimethyl sulfoxide (DMSO) and 0.2 mM 1-phenyl-2-thiourea (PTU) as previously described35. For Stat3 inhibitor experiments, larvae were treated with 7 µM JSI-124 (also known as Cucurbitacin I;
WebLiver is one of the largest internal organs in the body and its importance for metabolism, detoxification and homeostasis has been well established. Web24 Oct 2015 · Hepatocyte ablation was performed by treating Tg(fabp10a:CFP-NTR) larvae with 10 mM metronidazole (Mtz) in egg water supplemented with 0.2% dimethyl sulfoxide (DMSO). For chemical screening, the larvae were treated with 44 com-pounds from A0 h to R24 h or from R0 h to R48 h, and fabp10a:DsRed expression
WebThe Tg(fabp10a:CFP-NTR) adult fish were treated with 5 mmol/L Mtz only for 5 hours and subsequently treated with MS-275 from R19h to R4d. As observed in the larvae, Bhmt expression was greatly reduced and sox9b expression was significantly up-regulated in MS-275–treated regenerating adult livers compared with control regenerating livers ( Figure 4 …
Web26 Aug 2024 · The Tg(fabp10a:CFP-NTR) line expresses bacterial nitroreductase (NTR) fused with cyan fluorescent protein (CFP) under the hepatocyte-specific fabp10a promoter. NTR converts metronidazole (Mtz) into a cytotoxic drug; thus, Mtz treatment ablates cells that express NTR [20, 21] (e.g., hepatocytes in Tg[fabp10a:CFP-NTR] larvae). red192Web14 Aug 2024 · In Tg(fabp10a:pt-β-catenin) zebrafish larvae, ... Our group developed the Tg(fabp10a:CFP-NTR) zebrafish line that expresses nitroreductase (NTR) under the … know cell phone numberWebGeneration of the Tg(fabp10a:rtTA,TRE:Venus-KRAS) line . Gateway cloning (Thermo fisher Scientific) was used to make the fabp10a:rtTA-pA,TRE:CFP-pA construct with three entry … red1chiptuningWebTowards this aim, we have established a zebrafish model for hepatocyte-to-BEC conversion by employing Tg(fabp10a:CFP-NTR)zebrafish with a temporal inhibition of Notch signaling during regeneration. Cre/loxP-mediated permanent and H2B-mCherry-mediated short-term lineage tracing revealed that in the model, all BECs originate from hepatocytes. know cell phone number ownerWebBackground and aims: Injury to biliary epithelial cells (BECs) lining... know challanWebMethods: Using Tg (fabp10a:CFP-NTR) zebrafish, we examined the effects of 44 selected compounds on BEC-driven liver regeneration. Liver size was assessed by fabp10a:DsRed … red2000.comWebFollowing pharmacogenetic ablation of hepatocytes in Tg(fabp10a:CFP-NTR) zebrafish, resulting in severe liver injury, signal transducer and activator of transcription 3 (Stat3) and its target gene and negative regulator, socs3a, were upregulated in regenerating livers. red1974 o2.pl